Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: Synthesis of poly(HPMA)-g-CGGBeta11 graft copolymer and its self-assembly into a hydrogel via association of pendant β-sheet peptide strands.

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques:

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: A. Temperature-dependent CD spectra of Beta11 peptide (the arrow indicates the change of spectrum upon temperature increase); B. Temperature-dependent CD spectra of poly(HPMA)-g-CGGBeta11 graft copolymer; C. Temperature effect on the secondary structure of Beta11 and poly(HPMA)-g-CGGBeta11, recorded as change in ellipticity at 218 nm; D. pH-Dependent CD spectra of Beta11 peptide; E. pH-Dependent CD spectra of poly(HPMA)-g-CGGBeta11 graft copolymer.

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques:

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: FTIR spectra (solid) and second-derivatives (dotted) of A. Beta11 peptide; B. poly(HPMA)-g-CGGBeta11 graft copolymer.

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques:

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: A. Fluorescence emission spectra (λexc = 440 nm) of Beta11 peptide and poly(HPMA)-g-CGGBeta11 graft copolymer after addition of 50 µM ThT. ThT alone was used as blank; B. CR binding assay; C. Differential spectra of (Beta11/CR)-CR and (poly(HPMA)-g-CGGBeta11/CR)-CR showing the points of maximum absorption.

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques: Fluorescence, Binding Assay

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: A. TEM image of negatively stained Beta11 fibrils; B. TEM image of negatively stained poly(HPMA)-g-CGGBeta11 fibrils; C. SEM image of freeze-dried 0.4 wt % Beta11 gel; D. SEM image of freeze-dried 0.4 wt % poly(HPMA)-g-CGGBeta11 gel (the inset is the image of the cross-sectioned copolymer gel).

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques: Staining

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: Mean square displacement (MSD) as a function of lag time for 0.52 µm amidine-modified PS particles in water solutions/gels at different concentrations of A. Beta11 peptide; B. poly(HPMA)-g-CGGBeta11 graft copolymer.

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques: Modification

Journal:

Article Title: Self-Assembled Hydrogels from Poly[ N -(2-hydroxypropyl)methacrylamide] Grafted with ?-Sheet Peptides

doi: 10.1021/bm9005084

Figure Lengend Snippet: Frequency-dependent linear viscoelastic moduli for 0.52 µm amidine-modified PS particles in water solutions/gels of A. Beta11 peptide (solid symbols for loss modulus, G”, and half-open red symbols for storage modulus, G’); B. poly(HPMA)-g-CGGBeta11 graft copolymer (open symbols for loss modulus, G”, and half-solid red symbols for storage modulus, G’).

Article Snippet: The number-average molecular weight and molecular weight distribution of the copolymer were measured by size exclusion chromatography (SEC) on an ÄKTA FPLC system (Amersham Pharmacia Biotech) equipped with UV and RI detectors, using a Superose 6 column, previously calibrated with poly(HPMA) fractions, and phosphate buffer solution (PBS, pH = 7.2) as eluent.

Techniques: Modification